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Research Interest:
Cell Culture Bioengineering.細胞生物工程>Animal
cell culture can be used as an in vitro factory system
for producing many biologicals such as viral vaccines,
therapeutic proteins and monoclonal antibodies, tissue
cells and gene therapy vectors. These products usually
can not be generated effectively from bacteria or yeasts,
since many post-translational modifications, processing,
and complicate host cell interactions are required to present
their authentic biological functions.
Our interests are currently focused
on the cell culture-derived viral vaccines for Japanese encephalitis
virus, enterovirus type 71, dengue viruses (based on the cDNA
infectious clone) and the gene therapy-based adenovirus, adeno-associated
virus, retrovirus and lentivirus vectors. The other area is to
study the in vitro microevolution of RNA viruses in cell culture
system. We are currently using JEV and Dengue viruses as examples
to elucidate the in-vitro evolution and the RNA viruses. Our
studies can provide detailed information of investigating the
dynamics of heterogeneous virus populations in cell culture systems
that are of great concerns to vaccine development and manufacturing.
Molecular Viral Vaccines. 分子病毒疫苗Vaccination
as the medical procedure to protect pathogenic agent infection
has been a great achievement in human medicines. Example of small
pox vaccine has demonstrated a complete eradication of natural
pathogen to be possible through an effective vaccination program.
Our research is emphasized on applied virus biology studies including
viral epitope identification, viral interactions with host immune
system, viral virulence, and viral pathogenesis, which are important
to develop effective viral vaccines. Current interests are aimed
at understanding the neutralizing epitope(s) of flavivirus envelope
(E) protein and the interactions with host immune system. The
flavivirus E protein is the major target of neutralizing antibodies
produced by the host and is sufficient to elicit a protective
immune responses. We have successfully identified some of the
conformational epitopes with potentiality to induce anti-JEV
neutralizing antibodies.
Selected Publications:
- 1. Hu AY, Weng TC, Tseng YF, Chen YS, Wu CH, Hsiao S, Chou AH, Chao HJ, Gu A, Wu SC, Chong P, and Lee MS (2008). Microcarrier-based MDCK cell culture system for the production of influenza H5N1 vaccines. Vaccine 2008 Aug 28. [Epub ahead of print]
- WuSC*, Hong WW, and Liu JH (2008). Short hairpin RNA targeted to dihydrofolate reductase enhances the immunoglobulin G expression in gene-amplified stable Chinese hamster ovary cells. Vaccine 26(38), 4969-74.
- Liu CC, Lee SC, Butler M, and WuSC* (2008). High genetic stability of dengue virus propagated in MRC-5 cells as compared to the virus propagated in Vero cells. PLoS ONE 3(3), e1810.
- Cheng JW, Wu CW, Lin YT, Her SY, Huang KC, Lee MC, and WuSC (2007). Identification of the neutralizing antibody and heparin binding sites of the domain III of JEV and DENV envelope proteins. Biopolymers 88, 541-541.
- Hong WW, and WuSC*.(2007). A novel RNA silencing vector to improve antigen expression and stability in Chinese hamster ovary cells. Vaccine 25(20), 4103-4111.
- Liu CC, Lian WC, Butler M, and WuSC (2007). High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture. Vaccine 25, 19-24.
- Hong WWL, Yen YH, and WuSC (2007). Enhanced antibody affinity to Japanese encephalitis virus E protein by phage display. Biochemical and Biophysical Research Communications 356, 124-128.
- WuSC*,Yu JC, Hsu SH, and ChenDC (2006). Artificial Extracellular Matrix Proteins Contain Heparin-Binding and RGD-Containing Domains to Improve Osteoblast-like Cell Attachment and Growth. J Biomed Mater Res A79(3), 557-65.
- WuSC*, Liu JH, and Hong W (2006). Propagation kinetics of retrovirus transgene vector and replication-competent retrovirus in static and microcarrier cell culture systems using different medium exchange strategies. Enzyme and Microbial Technology 38, 229-236.
- Chin LC, Lai YK, WuSC, Lin CC, and Guo JH (2006). Production by Clonostachy compactiuscula of a Lovastatin Esterase That Converts Lovastatin to Monacolin J. Enzyme and Microbial Technology 39, 1051-1059.
- Chang CY, Hong W, Chong P, andWuSC* (2006). Influence of intron and exon splicing enhancers on mammalian cell expression of a truncated spike protein of SARS-CoV and its implication for subunit vaccine development. Vaccine24(8), 1132-41.
- Lin YJ, and WuSC* (2005). Histidine at residue 99 and the transmembrane region of the precursor membrane prM protein are important for the prM-E heterodimeric complex formation of Japanese encephalitis virus. J Virol. 79(13), 8535-44.
- Lin CW, andWuSC (2004). Identification of Mimotopes of the Japanese Encephalitis Virus Envelope Protein using Phage-Displayed Combinatorial Peptide Library. J Mol Microbiol Biotechnol8(1):34-42.
- WuSC*, Liu CC, and Lian WC (2004). Optimization of microcarrier cell culture for the inactivated enterovirus 71 vaccine development. Vaccine22, 3858-3864.
- WuSC*, Lin YJ, Chou JW, and Lin CW (2004). Construction and characterization of a Fab recombinant protein for Japanese encephalitis virus neutralization. Vaccine 23, 163-171.
- WuSC*, Chiang JR, and Lin CW (2004). Novel cell adhesive glycosaminoglycan-binding proteins of Japanese encephalitis virus. Biomacromolecules 5(6), 2160-2164.
- Wu SC*, Huang H, Lin CC (2004) Expression and
functional characterization of Helicobacter pylori catalase in
baculovirus-infected insect cells. Enzyme and Microbial Technology 35 (2004) 482–487.
- Liu JH, Wu SC, Chen WS, Yen CC, Yang MW, Tsai
YC, Chen PM (2004) An Intron Promotes the Anti-bcr-abl Activities
of a Retrovirally-Expressed Ribozyme in Chronic Myeloid Leukemia
Cells. Biochemical and Biophysical Research Communications 318,
764-772.
- Liu CC, Wu SC* (2004) Mosquito and mammalian cells
grown on microcarriers for four-serotype dengue virus production:
variations in virus titer, plaque morphology, and replication
rate. Biotechnology and Bioengineering 85(5):482-8.
- Wu SC*, Yu CH, Lin CW, Chu IM (2003) The domain
III fragment of Japanese encephalitis virus envelope protein:
mouse immunogenicity and liposome adjuvanticity. Vaccine 21,
2516-1522.
- Lin CW, Wu SC* (2003) A functional epitope determinant
on domain III of the Japanese encephalitis virus envelope protein
interacted with the neutralizing antibody combining sites. Journal
of Virology 77, 2600-2606.
- Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW,
Wu SC, Cheng JW. (2003) Structural basis of a flavivirus recognized
by its neutralizing antibody: solution structure of the domain
III of the Japanese encephalitis virus envelope protein. Journal
of Biological Chemistry 278:46007-13.
- Wu SC*, Lin CW, Lee SC, Lian WC (2003) Phenotypic
and genotypic characterization of the neurovirulence and neuroinvasiveness
of a large-plaque attenuated Japanese encephalitis virus isolate. Microbes and Infection 5, 475-480.
- Wu SC*, Lin YJ, Yu CH (2003) Baculovirus-insect
cell expression, purification, and immunological studies of the
full-length Japanese encephalitis virus envelope protein. Enzyme
and Microbial Technology 33, 438-444.
- Wu SC*, Chu IM (2003) Editorial: Special issue
on the 2002 symposium of Young Asian Biochemical Engineers' Community
(YABEC), Taipei, Taiwan. Enzyme and Microbial Technology 33,
331.
- Wu, S.-C.*, Huang, G.Y.-L., Liu, J.-H. (2002)
Production of retrovirus and adenovirus vectors for gene therapy:
a comparative study using microcarrier and stationary cell culture.
Biotechnology Progress 18, 617-622.
- Wu, S.-C.*, Huang, G.Y.-L. (2002) Stationary and
microcarrier cell culture processes for propagating Japanese
encephalitis virus. Biotechnology Progress 18, 124-8.
- Wu, S.-C.*, C.-W. Lin (2001) Neutralizing peptide
ligands selected from phage displayed libraries mimic the conformational
epitope on domain III of the Japanese encephalitis virus envelope
protein. Virus Research 76, 59-69.
- Wu, S.-C.*, S.-C. Lee (2001) Complete nucleotide
sequences and cell-line multiplication patterns of the attenuated
variant CH2195LA of Japanese encephalitis virus. Virus Research 73, 91-102.
- Wu, S.-C.*, G. Y.-L. Huang (2000) Hydrodynamic
shear forces increase Japanese encephalitis virus production
from microcarrier-grown Vero cells. Bioprocess Engineering 23,
229-233.
- Lin, C.-W., S.-C. Wu, S.-C. Lee, K.-S. Cheng (2000)
Genetic analysis and clinical evaluation of vaculoating cytotoxin
gene A and cytotoxin-associated gene A in Taiwanese Helicobacter
pylori isolates from peptic ulcer patients. Scan. Journal of
Infectious Diseases 32, 51-57.
- Wu, S.-C.* (1999) Inflence of hydrodynamic shear
stress on microcarrier-attached cell growth: cell line dependency
and surfactant protection. Bioprocess Engineering 21, 201-206.
- 吳夙欽 (1999) 動物細胞工業與微載體培養技術的發展 生物產業 10(2), 113-117.
- 吳夙欽 (2002) 利用基因工程技術與昆蟲細胞培養來生產日本腦炎病毒套膜蛋白 工程科技通訊 第62期64-44.
- 劉家齊 洪偉立 吳夙欽 (2003) 動物細胞產業與生化工程技術的發展 化工技術 第11卷第5期208-220.
- 吳夙欽 (2003) 第十章 動物細胞培養 生物產業技術概論 國立清華大學出版社 301-314.
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