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生物巨分子晶體結構實驗室成果

我們實驗室利用本系(清華大學生命科學系)的蛋白質純化與表達核心設施 (細胞打碎機、FPLC、HPLC等)，大量分離純化我們所需的蛋白質樣品，並使用本系公用蛋白質定性儀器(旋光儀、恆溫滴定熱卡計、質譜儀、分析級超高速離心機等)，決定蛋白質的生物功能。我們利用本系生物巨分子Ｘ光繞射儀核心實驗室的Ｘ光繞射儀，收集蛋白質晶體繞射數據。我們也充分地利用了臺灣新竹同步幅射研究中心 (NSRRC) 之的光源、設備及日本同步幅射研究中心 (SP8)之設備。截至目前，本實驗室決定了數個蛋白質的三度空間結構，如表一所示。對同步幅射光源也具備豐富的使用經驗及操作能力

Table 1a. Crystal structures of proteins from Helicobacter pylori determined by X-ray crystallography in our laboratory

Crystal
3D Structure
Name	HP0620	HP0832	HP0242
Function	Inorganic pyrophosphatase Hydrolyzes inorganic pyrophosphate to orthophosphates	Spermidine synthase Catalyzes the transfer of the propylamine moiety to putrescine in the biosynthesis of spermidine	Hypothetical protein Novel folding
Reference	To be Published	To be Published	To be Published
Year	2004	2004	2004

Table 1b. Crystal structures of different kind of proteins determined by X-ray crystallography and published in our laboratory

Crystal
3D Structure
Name	1UVA	1UVB	1UVC
Function	rice ns-LTP1 Transfer lipids between membranes
Reference	Protein Sci. 13 pp. 2304	Protein Sci. 13 pp. 2304	Protein Sci. 13 pp. 2304
Year	2004	2004	2004

Crystal
3D Structure
Name	1OJ1	1OJ8	1H65	1E9L
Function	Ribonuclease Degrade RNA		Pea Toc34 Protein transporter across the chloroplast outer membrane	Lectin Ym1 Mediates cell-cell interactions and carbohydrate recognition
Reference	To be Published	To be Published	Nat.Struct.Biol. 9 pp. 95	J.B.C. 276 pp. 17507
Year	2003	2003	2002	2001